In this volume contemporary methods designed to provide insights into, mathematical structure for, and predictive inferences about neuroendocrine control mechanisms are presented.Key Features* Collates an array of contemporary techniques for analysis of neuroendocrine data* Discusses current problems in and solutions to neurohormone pulse analysis* Identifies relevant software available..

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike.Comprehensive protocols included for:* Enzymes involved in the activation of bioactive peptidases and proteins* Prohormone/neuropeptide processing pathways* Enzymes involved in peptide metabolism* Posttranslational processing enzymes..

This volume includes paradigms, model systems, and techniques for the study of dysfunctions in the nervous system. The advantages and disadvantages of the approaches presented are critically discussed.Key Features* Neural injury* Developmental cell death* Disease processes and aging..

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological Macromolecules, Part C is written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences..

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.Key Features* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies* Includes overviews of* Optical probes and reagents* Fabrication and use of ion-selective microelectrodes* Use of NMR spectroscopy* Ionophores..

In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses...

G Proteins..

Bone disease, particularly osteoporosis, has emerged as a common and serious complication of solid organ transplantation. In recent years there have been real advances in our understanding of the pathogenesis and pathophysiology of bone loss, however treatment studies have been relatively sparse and successful strategies to reduce skeletal morbidity after transplantation remain to be clearly established. This book provides a unique resource for the many health professionals involved with transplantation of bone disease, both in terms of its scientific background and the management of the disease in clinical practice.Contents:-Basic Transplantation and Bone Biology-Pathogenesis of Transplantation Related Bone Disease-Clinical Features of Transplantation Bone Disease-Management..

The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies.* Presents detailed protocols* Includes troubleshooting tips* Provides coverage on structural biology, computational methods, and biology..

This volume continues the in-depth treatment of the topic and covers the RSG protein superfamily including RZ, R4, R7, R12, RhoGEF, and GRK, as well as other heterotrimeric G-protein signaling regulators.Table of Contents-RZ Subfamily-R4 Subfamily-R7 Subfamily-R12 Subfamily-RhoGEF Subfamily-GRK Subfamily-Other RGS proteins-Activators-Inhibitors-Other Modulators..

This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.Table of Contents-Expression and post-translational modification-Assays of GAP activity and allosteric control-Electrophysiological methods and RGS-insenstitive Ga subunits-Mouse Models of RGS protein action-Methods of RGS protein inhibition-G-protein regulators of model organisms..

Quinones are members of a class of aromatic compounds with two oxygen atoms bonded to the ring as carbonyl groups. This volume covers more clinical aspects of quinines, such as anticancer properties, as well as their role in nutrition and in age-related diseases.*Mitochondrial Ubiquinone and Reductases*Anticancer Quinones and Quinone Oxido-Reductases*Quininone Reductases: Chemoprevention, Nutrition*Quinones and Age-Related Diseases..

This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.Table of Contents-Methodology-Applications-Opzimization and Screening-Applications-Directed Evolution of Enzymatic Function-Applications-Evolution of Biosynthetic Pathways-Devices, Antibodies and Vaccines..

The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields...

The contributions in this volume emphasize analysis of experimental data and analytical biochemistry, with examples taken from biochemistry. They serve to inform biomedical researchers of the modern data analysis methods that have developed concomitantly with computer hardware.Selected Contents: A practical approach to interpretation of SVD results; modeling of oscillations in endocrine networks with feedback; quantifying asynchronous breathing; sample entropy; wavelet modeling and processing of nasal airflow traces..

The aim of this volume is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected.Selected Contents: Prediction of protein structure; modeling and studying proteins with molecular dynamics; statistical error in isothermal titration calorimetry; analysis of circular dichroism data; model comparison methods..