Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels of light continue to push the limits of detection, allowing ready monitoring in real time of intricate subcellular processes within living cells. This book affords a glimpse of the state of the art of a rapidly advancing field, and presents to users of these methods a detailed reference of current activities in the field.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.Key Features@bul* Outlines the use of luminescent reporter technologies to monitor gene expression and protein trafficking* Describes the luminescence-based clinical assay technologies* Details the basic biochemistry, biophysics, and chemistry of light-emitting reactions that are critical for applications* Includes explanations of the instrumentation used for detection and quantification of low level light* Shows the new applications of luminescence-based technologies that result largely from broad advances in recombinant DNA technologies and nonscale methods..

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences...

This volume of Current Topics in Developmental Biology showcases the research and therapeutic value of stem cells, and is proof of the increasing maturation of the fields of regenerative and molecular medicine. Reviews include:* Therapeutic cloning and tissue engineering to produce functional replacement tissues.* The role of the protein a-synuclein in a host of neurodegenerative diseases, which may be the first to benefit from stem cell therapy.* The curious array of functions in eukaryotic DNA methyltransferase, crucial for normal development and at the core of epigenetics regulation.* Influencing stem cell differentiation with mechanical stress, another example of genes versus the environment.* The process of mammalian hematopoeisis, and inducing it in model organisms with embryonic stem cells.* The regulation of differential parental germline genetic imprinting, central for epigenetics. * The molecular function and regulation of the cystic fibrosis gene, critical for understanding the complete molecular mechanism of this devastating disorder, which might soon be treatable with stem cells.This volume comprehensively describes some of the most current issues in stem cell biology, and is an exciting preview of therapies that may soon be applied in the clinic. It is essential reading for researchers, clinicians, and trainees alike.* Therapeutic Cloning and Tissue Engineering* Structure and function of eukaryotic DNA methyltransferases* Mechanical Signals as Regulators of Stem Cell Fate* Molecular basis for the Chloride Channel Activity of CFTR and the Consequences of Disease Causing Mutations..

This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion.Key Features* world-renowned experts* state-of-the-art experimental methods* monolayers, bilayers, biological membranes * theoretical aspects and computer simulations* rafts* synaptic transmission* membrane fusion* signal transduction..

Purinergic receptors are proteins that bind ATP as their extracellular ligand. Once thought only as an intracellular molecule that provides energy, ATP is also now considered an essential autocrine/paracrine agonist that acts extracellularly within tissues and tissue microenvironments. Receptors for ATP and its metabolites, so-called "purinergic receptors," are essential membrane receptors that transduce the extracellular signal carried by ATP. Many cell types or tissues express multiple types of these receptors, often on the same cell. This volume will cover the history and overall impact of extracellular ATP signaling, methods to detect ATP release and signaling, the molecular and cell biology of purinergic receptors, the pharmacology of purinergic receptors, the ion channel biophysics and biochemistry of P2X purinergic receptor channels, and the physiology and pathophysiology of purinergic receptors.Key Features* Organized and written by world-renowned experts* Covers state-of-the-art experimental methods* Has broad appeal to basic and clinical scientists, the biotch industry and pharmaceutical research companies..

The Internet is connecting enterprises into a global economy. Companies are exposing their directories, or a part of their directories, to customers, business partners, the Internet as a whole, and to potential "hackers." If the directory structure is compromised, then the whole enterprise can be at risk. Security of this information is of utmost importance.This book provides examples and implementation guidelines on building secure and structured enterprise directories. The authors have worked with corporations around the world to help them design and manage enterprise directories that operate efficiently and guard against outside intrusion. These experts provide the reader with "best practices" on directory architecture, implementation, and enterprise security strategies...

Current Topics in Developmental Biology provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology. This volume contains ten important contributions from leading minds in developmental biology.* Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science * Presents major contemporary issues and astonishing discoveries at the forefront of modern developmental biology, stem cells and cloning, and regenerative medicine* The longest-running forum for current issues in developmental biology with over 30 years of coverage..

Together with other volumes in this series, Volume 56 presents thoughtful and forward-looking articles on developmental biology and developmental medicine. Reviews include:* Selfishness in moderation: evolutionary success of the yeast plasmid* Nongenomic actions of androgen in sertoli cells* Regulation of chromatin structure and gene activity by Poly(ADP-ribose) polymerases* Centromeres and Kinetochores, Who Needs 'Em? The Role of Non-centromeric Chromatin in Spindle Assembly* Modeling Cardiogenesis: The Challenges and Promises of 3D Reconstruction* Plasmid and Chromosome Traffic Control: How ParA and ParB Drive PartitionThe exceptional reviews in this volume of Current Topics in Developmental Biology will be valuable to both clinical and fundamental researchers, as well as students and other professionals who want an introduction to current topics in cellular and molecular approaches to developmental biology and clinical problems of aberrant development.* Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science* Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine* The longest-running forum for contemporary issues in developmental biology with over 30 years of coverage..

In keeping with the tradition of excellence of past volumes of Current Topics in Developmental Biology, this volume provides a comprehensive survey of major issues at the forefront of modern developmental biology and developmental medicine. Astonishing discoveries are presented in seven thoughtful and forward-looking articles.* The Balbiani body and germ cell determinants: 150 years later* Fetal-maternal interactions: Prenatal psychobiological precursors to adaptive infant development* The Paradoxical Role of Methyl-CpG-Binding Protein 2 (MeCP2) in Rett Syndrome* Genetic Approaches to Analyzing Mitochondrial Outer Membrane Permeability* Mitochondrial dynamics in mammals* Histone Modifications in Corepressor Functions* Death by Abl, A Matter of LocationClinical and fundamental researchers, as well as students and other professionals, will find this series an indispensable guide to current topics in cellular and molecular approaches to developmental biology and clinical problems of aberrant development.* Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science* Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine* The longest-running forum for contemporary issues in developmental biology with over 30 years of coverage..

Current Topics in Developmental Biology provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology. This volume contains eight important contributions from leading minds in developmental biology.* Hepatic Oval Cells: Helping Redefine a Paradigm in Stem Cell Biology * Meiotic DNA Replication * Pollen Tube Guidance: the Role of Adhesion and Chemotropic Molecules * The biology and diagnostic applications of fetal DNA and RNA in maternal plasma * Advances in Tissue Engineering * Directions in cell migration along the rostral migratory stream: the pathway for migration in the brain * Retinoids in Lung Development and Regeneration * Structural Organization and Functions of the Nucleus in Development, Aging and Disease* Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science * Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine * The longest-running forum for contemporary issues in developmental biology with over 30 years of coverage..

Current Topics in Developmental Biology provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology. This volume contains six important contributions from leading minds in developmental biology.* Presents major contemporary issues and astonishing discoveries at the forefront of modern developmental biology, stem cells, cloning, and regenerative medicine * Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science * The longest-running forum for current issues in developmental biology with over 30 years of coverage..

Current Topics in Developmental Biology provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology. This volume contains nine important contributions from leading minds in developmental biology.* Presents major contemporary issues and astonishing discoveries at the forefront of modern developmental biology, stem cells, cloning, and regenerative medicine * Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science * The longest-running forum for current issues in developmental biology with over 30 years of coverage..

The global credit derivatives market is estimated to have grown from virtually nothing in the early 1990s to over $2 trillion dollars. Although still relatively young, the credit derivatives market has already developed to the point where one can characterize its evolution in terms of developments in its various segments, such as the market for single-name credit derivatives or the market for credit derivatives written on sovereign credits.Understanding Credit Derivatives offers a comprehensive introduction to the credit derivatives market. Rather than presenting a highly technical exploration of the subject, it offers intuitive and rigorous summaries of the major subjects and the principal perspectives associated with them. The centerpiece is pricing and valuation issues, especially discussions of different valuation tools and their use in credit models.* Offers a broad overview of this growing field * Discusses all the main types of credit derivatives * Provides back-of-the-book summary of statistics and fixed-income mathematics..

Fiduciary responsibilities and related court-imposed liabilities have forced investors to assess market conditions beyond gut level, resulting in the development of sophisticated decision-making tools. Roger Brown's use of historical real estate data enables him to develop tools for gauging the impact of circumstances on relative risk. His application of higher level statistical modeling to various aspects of real estate makes this book an essential partner in real estate research. Offering tools to enhance decision-making for consumers and researchers in market economies of any country interested in land use and real estate investment, his book will improve real estate market efficiency. With property the world's biggest asset class, timely data on housing prices just got easier to find and use.*Excellent mixture of theory and application*Data and database analysis techniques are the first of their kind*CDROM contains pre-written code for data analysis tailored specifically to real estate settings..

Drawing together literature from a variety of fields, Food Texture and Viscosity, 2E, includes a brief history of this area and its basic principles. It reviews how texture and viscosity are measured, including the physical interactions between the human body and food, objective methods of texture measurements, the latest advances in texture-measuring instruments, various types of liquid flow, and more.This revised edition contains approximately 30% new material, including two new chapters on physics and texture and the correlation between physical measurements and sensory assessments. It now includes two-color illustrations and includes a current list of equipment suppliers.* Completely revised with approximately 30% new material * Includes two new chapters on physics and texture and the correlation between physical measurements and sensory assessments* Provides a list of suppliers of texture-measuring equipment * Features two-color illustrations and text throughout* Written by an award-winning author..

Decision Methods for Forest Resource Management focuses on decision making for forests that are managed for both ecological and economic objectives. The essential modern decision methods used in the scientific management of forests are described using basic algebra, computer spreadsheets, and numerous examples and applications. Balanced treatment is given throughout the book to the ecological and economic impacts of alternative management decisions in both even-aged and uneven-aged forests.* In-depth coverage of both ecological and economic issues* Hands-on examples with Excel spreadsheets; electronic versions available on the authors' website* Many related exercises with solutions* Instructor's Manual available upon request..